Justin Timberlake and Jimmy Fallon were at it again on last night's episode of Late Night.

The last time Justin Timberlake visited Jimmy Fallon on Late Night, the two performed a hilarious medley of old school hip-hop songs in "History of Rap". The video of that performance went viral almost immediately. Timberlake and Fallon brought the routine back last night, much to the delight of the studio audience and those of us watching at home. Fallon and Timberlake picked up where they left off (in the late 80's) and covered the past twenty years of rap with parts of hits by Public Enemy, N.W.A., Salt-n-Pepa, Cypress Hill, DMX, Outkast, 50 Cent, Lil Wayne and Rick Ross. The sing along version of Biz Markie's "Just A Friend" is amazing, and the audience was really into it. But, current major rap icons like Eminem, Jay-Z, Snoop Dogg, Kanye West, and the Beastie Boys weren't included. Check out the video after the jump!

Maybe there's a "History of Rap, Take 3" in the works? We can only hope!